A highly sensitive method for the determination of chlorpyrifos in vegetable and water samples at Abhanpur area

Abstract

 A new and highly sensitive spectrophotometric method for the determination of widely used organophosphorus insecticide chlorpyrifos. The method is based on alkaline hydrolysis of chlorpyrifos to 1,2,4 trichloropyridine, followed by coupling with 3, 4 dimethyl aniline reagent in alkaline medium. The absorption maxima of the yellowish-orange dye formed is measured at 460 nm. Beer’s law is obeyed over the concentration range of 6 to 42µg in a final solution volume of 25 ml (0.16 - 1.68ppm). The molar absorptivity, sandell’s sensitivity, correlation coefficient have been determined. The method is simple sensitive and free from interferences of other pesticides and diverse ions. The method has been satisfactorily applied to the determination of chlorpyrfos in different environmental and biological samples.