To Investigate the Effects of Microrna-433-3P on Proliferation, Migration, Invasion and Apoptosis of Lung Cancer Cells and Its Molecular Mechanism
Non-small cell lung cancer (NSCLC) is one of the most important types of lung cancer (LC) causing higher death rate. Despite the fact that microRNA 433 (miR 433) has been discovered to be downregulated in a variety of human cancers, more research on its expression, roles, and biological processes in NSCLC is required. In this work, we attempted to identify the microRNA‑433-3p (miR-433-3p) expression and evaluate its impacts on multiplication, motility, invasion and apoptosis of NSCLC cells in NSCLC. NSCLC cell lines were obtained and the RT-qPCR (reverse transcription‑quantitative polymerase chain reaction) was carried out to find the expression of suggested microRNA in NSCLC. To study the impacts of miR-433-3p on NSCLC regulation process, NSCLC cell lines were transfected with miR-433 mimics. The impacts of microRNA-433 on NSCLC proliferation, motility, invasion and apoptosis were examined by Cell Counting Kit (CCK), WH (Wound Healing) Test, Transwell (TW) Test, and FC (flow cytometry) respectively. The findings demonstrated that microRNA‑433-3P was considerably downregulated in NSCLC. In addition, the results illustrated that miR-433-3p has minimized the rate of multiplication, motility, and invasion and enhanced apoptosis in NSCLC. Hence, miR-433-3p may be viewed as new targets for the discovery of successful treatment strategies for NSCLC patients.