Biodiesel Production by the Purified Lipase as Biocatalytic from Pseudomonas Putida Isolated from Oil-Contaminated Soil

Abstract

Biodiesel is a viable alternative fuel since it is both renewable and beneficial to the environment. A more stable catalyst, enzymes are easier to make and safer to use in the manufacture of biodiesel. Biodiesel was made with Pseudomonas putida as a feedstock.maximum lipolytic activity for oils revealed by Pseudomonas putida followed by Pseudomonas aeruginosa then Serratia marcesences. In contrast, lower lipolytic activity presented by Acinetobacter baumanniiisolates.The corn oil was the best inducer for lipase after 48hours  that reached to 33 U/ml followed by egg yolk suspension with 28U/ml. The lipase was purified by 55% saturation of ammonium sulfate and DEAE- cellulose  column followed by sephadex G-100 with final recovery of 42%. and 5.9 fold of purification.The transesterification reaction was carried out with methanol and different catalyst as NaOH, KOH and lipase. The percentage of FAA yields were 60%, 81% and 97%, respectively. Also the results revealed that oleic acid was present in higher percentage 33% in case of using the lipase as biocatalyst and linoleic acid with lower percentage 11% .Because of this, the lipase from Pseudomonas putida is a great choice for testing biofuel generation at industrial scale.